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Science 289 (5484): 1550-1554

Copyright © 2000 by the American Association for the Advancement of Science

Selective Inhibition of NF-kappa B Activation by a Peptide That Blocks the Interaction of NEMO with the Ikappa B Kinase Complex

Michael J. May,1 Fulvio D'Acquisto,1 Lisa A. Madge,2 Judith Glöckner,1 Jordan S. Pober,2 Sankar Ghosh1*

Activation of the transcription factor nuclear factor (NF)-kappa B by proinflammatory stimuli leads to increased expression of genes involved in inflammation. Activation of NF-kappa B requires the activity of an inhibitor of kappa B (Ikappa B)-kinase (IKK) complex containing two kinases (IKKalpha and IKKbeta ) and the regulatory protein NEMO (NF-kappa B essential modifier). An amino-terminal alpha -helical region of NEMO associated with a carboxyl-terminal segment of IKKalpha and IKKbeta that we term the NEMO-binding domain (NBD). A cell-permeable NBD peptide blocked association of NEMO with the IKK complex and inhibited cytokine-induced NF-kappa B activation and NF-kappa B-dependent gene expression. The peptide also ameliorated inflammatory responses in two experimental mouse models of acute inflammation. The NBD provides a target for the development of drugs that would block proinflammatory activation of the IKK complex without inhibiting basal NF-kappa B activity.

1 Section of Immunobiology and Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06510, USA.
2 Interdepartmental Program in Vascular Biology and Transplantation, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, CT 06536, USA.
*   To whom correspondence should be addressed. E-mail: sankar.ghosh{at}yale.edu

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   Abstract »    Full Text »    PDF »
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Host-Adapted Borrelia burgdorferi in Mice Expresses OspA during Inflammation.
H. Crowley and B. T. Huber (2003)
Infect. Immun. 71, 4003-4010
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Glycogen Synthase Kinase 3{beta}-Mediated Apoptosis of Primary Cortical Astrocytes Involves Inhibition of Nuclear Factor {kappa}B Signaling.
J. F. Sanchez, L. F. Sniderhan, A. L. Williamson, S. Fan, S. Chakraborty-Sett, and S. B. Maggirwar (2003)
Mol. Cell. Biol. 23, 4649-4662
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In Vivo Identification of Inducible Phosphoacceptors in the IKK{gamma}/NEMO Subunit of Human I{kappa}B Kinase.
R. S. Carter, K. N. Pennington, B. J. Ungurait, and D. W. Ballard (2003)
J. Biol. Chem. 278, 19642-19648
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Mitochondria to nucleus stress signaling: a distinctive mechanism of NF{kappa}B/Rel activation through calcineurin-mediated inactivation of I{kappa}B{beta}.
G. Biswas, H. K. Anandatheerthavarada, M. Zaidi, and N. G. Avadhani (2003)
J. Cell Biol. 161, 507-519
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Tetrameric Oligomerization of I{kappa}B Kinase {gamma} (IKK{gamma}) Is Obligatory for IKK Complex Activity and NF-{kappa}B Activation.
S. Tegethoff, J. Behlke, and C. Scheidereit (2003)
Mol. Cell. Biol. 23, 2029-2041
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Protection of Islets by in Situ Peptide-mediated Transduction of the Ikappa B Kinase Inhibitor Nemo-binding Domain Peptide.
K. K. Rehman, S. Bertera, R. Bottino, A. N. Balamurugan, J. C. Mai, Z. Mi, M. Trucco, and P. D. Robbins (2003)
J. Biol. Chem. 278, 9862-9868
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Neurokinin 1 receptors and neprilysin modulation of mouse bladder gene regulation.
I. Dozmorov, M. R. Saban, N. P. Gerard, B. Lu, N.-B. Nguyen, M. Centola, and R. Saban (2003)
Physiol Genomics 12, 239-250
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