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Science 287 (5454): 869-873

Copyright © 2000 by the American Association for the Advancement of Science

A Subclass of Ras Proteins That Regulate the Degradation of Ikappa B

Craig Fenwick, 1* Soon-Young Na, 24* Reinhard E. Voll, 1 Haihong Zhong, 1 Suhn-Young Im, 35 Jae Woon Lee, 45dagger Sankar Ghosh 1dagger

Small guanosine triphosphatases, typified by the mammalian Ras proteins, play major roles in the regulation of numerous cellular pathways. A subclass of evolutionarily conserved Ras-like proteins was identified, members of which differ from other Ras proteins in containing amino acids at positions 12 and 61 that are similar to those present in the oncogenic forms of Ras. These proteins, kappa B-Ras1 and kappa B-Ras2, interact with the PEST domains of Ikappa Balpha and Ikappa Bbeta [inhibitors of the transcription factor nuclear factor kappa B (NF-kappa B)] and decrease their rate of degradation. In cells, kappa B-Ras proteins are associated only with NF-kappa B:Ikappa Bbeta complexes and therefore may provide an explanation for the slower rate of degradation of Ikappa Bbeta compared with Ikappa Balpha .

1 Section of Immunobiology and Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06510, USA.
2 Department of Biology,
3 Department of Microbiology,
4 Center for Ligand and Transcription and
5 Hormone Research Center, Chonnam National University, Kwangju 500-757, Korea.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed.

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