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Science 287 (5450): 142-144
Copyright © 2000 by the American Association for the Advancement of Science
Stat3-Mediated Transformation of NIH-3T3 Cells by the Constitutively Active Q205L G o Protein
Prahlad T. Ram,
1*
Curt M. Horvath,
2
Ravi Iyengar
1
Expression of Q205L G o
(Go*), an alpha subunit of heterotrimeric guanine
nucleotide-binding proteins (G proteins) that lacks guanosine triphosphatase (GTPase) activity in NIH-3T3 cells, results in
transformation. Expression of Go* in NIH-3T3 cells
activated signal transducer and activator of transcription 3 (Stat3)
but not mitogen-activated protein (MAP) kinases 1 or 2. Coexpression of
dominant negative Stat3 inhibited Go*-induced
transformation of NIH-3T3 cells and activation of endogenous Stat3.
Furthermore, Go* expression increased activity of the
tyrosine kinase c-Src, and the Go*-induced activation of
Stat3 was blocked by expression of Csk (carboxyl-terminal Src kinase),
which inactivates c-Src. The results indicate that Stat3 can function
as a downstream effector for Go* and mediate its
biological effects.
1 Department of Pharmacology,
2 Immunobiology Center, Mount Sinai School of
Medicine, One Gustave L. Levy Place, New York, NY 10029, USA.
*
To whom correspondence should be addressed. E-mail:
ramp01{at}doc.mssm.edu
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