Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
GoGreen Membership

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Logo for

J. Cell Biol. 150 (6): 1299-1310

Copyright © 2000 by the Rockefeller University Press.

© The Rockefeller University Press, /2000/9/1299/ $5.00
The Journal of Cell Biology, Volume 150, Number 6, September 18, 2000 1299-1310
Original Article Mechanism of N-WASP Activation by CDC42 and Phosphatidylinositol 4,5-bisphosphate Rajat Rohatgia, Hsin-yi Henry Hoa, and Marc W. Kirschnera a Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115 Marc W. Kirschner, Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115. Tel:(617) 432-2250 Fax:(617) 432-0420

Neuronal Wiskott-Aldrich Syndrome protein (N-WASP) transmits signals from Cdc42 to the nucleation of actin filaments by Arp2/3 complex. Although full-length N-WASP is a weak activator of Arp2/3 complex, its activity can be enhanced by upstream regulators such as Cdc42 and PI(4,5)P2. We dissected this activation reaction and found that the previously described physical interaction between the NH2-terminal domain and the COOH-terminal effector domain of N-WASP is a regulatory interaction because it can inhibit the actin nucleation activity of the effector domain by occluding the Arp2/3 binding site. This interaction between the NH2- and COOH termini must be intramolecular because in solution N-WASP is a monomer. Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) influences the activity of N-WASP through a conserved basic sequence element located near the Cdc42 binding site rather than through the WASp homology domain 1. Like Cdc42, PI(4,5)P2 reduces the affinity between the NH2- and COOH termini of the molecule. The use of a mutant N-WASP molecule lacking this basic stretch allowed us to delineate a signaling pathway in Xenopus extracts leading from PI(4,5)P2 to actin nucleation through Cdc42, N-WASP, and Arp2/3 complex. In this pathway, PI(4,5)P2 serves two functions: first, as an activator of N-WASP; and second, as an indirect activator of Cdc42.

phosphoinositides, Cdc42 GTP-binding protein, actin, signal transduction, Wiskott-Aldrich Syndrome protein

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Arp2/3 Activators WAVE and WASP Have Distinct Genetic Interactions With Rac GTPases in Caenorhabditis elegans Axon Guidance.
M. A. Shakir, K. Jiang, E. C. Struckhoff, R. S. Demarco, F. B. Patel, M. C. Soto, and E. A. Lundquist (2008)
Genetics 179, 1957-1971
   Abstract »    Full Text »    PDF »
The Cdc42 Effector IRSp53 Generates Filopodia by Coupling Membrane Protrusion with Actin Dynamics.
K. B. Lim, W. Bu, W. I. Goh, E. Koh, S. H. Ong, T. Pawson, T. Sudhaharan, and S. Ahmed (2008)
J. Biol. Chem. 283, 20454-20472
   Abstract »    Full Text »    PDF »
Regulation of Gic2 Localization and Function by Phosphatidylinositol 4,5-Bisphosphate during the Establishment of Cell Polarity in Budding Yeast.
K. Orlando, J. Zhang, X. Zhang, P. Yue, T. Chiang, E. Bi, and W. Guo (2008)
J. Biol. Chem. 283, 14205-14212
   Abstract »    Full Text »    PDF »
Regulation of Actin Assembly Associated With Protrusion and Adhesion in Cell Migration.
C. Le Clainche and M.-F. Carlier (2008)
Physiol Rev 88, 489-513
   Abstract »    Full Text »    PDF »
Membrane association and functional regulation of Sec3 by phospholipids and Cdc42.
X. Zhang, K. Orlando, B. He, F. Xi, J. Zhang, A. Zajac, and W. Guo (2008)
J. Cell Biol. 180, 145-158
   Abstract »    Full Text »    PDF »
The -amyloid protein of Alzheimer's disease increases neuronal CRMP-2 phosphorylation by a Rho-GTP mechanism.
S. Petratos, Q.-X. Li, A. J. George, X. Hou, M. L. Kerr, S. E. Unabia, I. Hatzinisiriou, D. Maksel, M.-I. Aguilar, and D. H. Small (2008)
Brain 131, 90-108
   Abstract »    Full Text »    PDF »
Identification of Novel Membrane-binding Domains in Multiple Yeast Cdc42 Effectors.
S. Takahashi and P. M. Pryciak (2007)
Mol. Biol. Cell 18, 4945-4956
   Abstract »    Full Text »    PDF »
ARF1-mediated actin polymerization produces movement of artificial vesicles.
J. Heuvingh, M. Franco, P. Chavrier, and C. Sykes (2007)
PNAS 104, 16928-16933
   Abstract »    Full Text »    PDF »
Rac1 and Rac2 differentially regulate actin free barbed end formation downstream of the fMLP receptor.
C. X. Sun, M. A.O. Magalhaes, and M. Glogauer (2007)
J. Cell Biol. 179, 239-245
   Abstract »    Full Text »    PDF »
Stimulation of Actin Polymerization by Vacuoles via Cdc42p-dependent Signaling.
S. Isgandarova, L. Jones, D. Forsberg, A. Loncar, J. Dawson, K. Tedrick, and G. Eitzen (2007)
J. Biol. Chem. 282, 30466-30475
   Abstract »    Full Text »    PDF »
c-Abl-mediated Phosphorylation of WAVE3 Is Required for Lamellipodia Formation and Cell Migration.
K. Sossey-Alaoui, X. Li, and J. K. Cowell (2007)
J. Biol. Chem. 282, 26257-26265
   Abstract »    Full Text »    PDF »
Unregulated actin polymerization by WASp causes defects of mitosis and cytokinesis in X-linked neutropenia.
D. A. Moulding, M. P. Blundell, D. G. Spiller, M. R.H. White, G. O. Cory, Y. Calle, H. Kempski, J. Sinclair, P. J. Ancliff, C. Kinnon, et al. (2007)
J. Exp. Med. 204, 2213-2224
   Abstract »    Full Text »    PDF »
An NGF-induced Exo70-TC10 complex locally antagonises Cdc42-mediated activation of N-WASP to modulate neurite outgrowth.
D. Pommereit and F. S. Wouters (2007)
J. Cell Sci. 120, 2694-2705
   Abstract »    Full Text »    PDF »
Phosphatidylinositol-4,5 Bisphosphate Produced by PIP5KI{gamma} Regulates Gelsolin, Actin Assembly, and Adhesion Strength of N-Cadherin Junctions.
T. Y. El Sayegh, P. D. Arora, K. Ling, C. Laschinger, P. A. Janmey, R. A. Anderson, and C. A. McCulloch (2007)
Mol. Biol. Cell 18, 3026-3038
   Abstract »    Full Text »    PDF »
Phosphorylation of a Wiscott-Aldrich Syndrome Protein-associated Signal Complex Is Critical in Osteoclast Bone Resorption.
M. A. Chellaiah, D. Kuppuswamy, L. Lasky, and S. Linder (2007)
J. Biol. Chem. 282, 10104-10116
   Abstract »    Full Text »    PDF »
IQGAP1 regulates cell motility by linking growth factor signaling to actin assembly.
L. B. Bensenor, H.-M. Kan, N. Wang, H. Wallrabe, L. A. Davidson, Y. Cai, D. A. Schafer, and G. S. Bloom (2007)
J. Cell Sci. 120, 658-669
   Abstract »    Full Text »    PDF »
IQGAP1 Stimulates Actin Assembly through the N-Wasp-Arp2/3 Pathway.
C. Le Clainche, D. Schlaepfer, A. Ferrari, M. Klingauf, K. Grohmanova, A. Veligodskiy, D. Didry, D. Le, C. Egile, M.-F. Carlier, et al. (2007)
J. Biol. Chem. 282, 426-435
   Abstract »    Full Text »    PDF »
Actin Polymerization Serves as a Membrane Domain Switch in Model Lipid Bilayers.
A. P. Liu and D. A. Fletcher (2006)
Biophys. J. 91, 4064-4070
   Abstract »    Full Text »    PDF »
Role of RacC for the Regulation of WASP and Phosphatidylinositol 3-Kinase during Chemotaxis of Dictyostelium.
J. W. Han, L. Leeper, F. Rivero, and C. Y. Chung (2006)
J. Biol. Chem. 281, 35224-35234
   Abstract »    Full Text »    PDF »
Defective Th1 Cytokine Gene Transcription in CD4+ and CD8+ T Cells from Wiskott-Aldrich Syndrome Patients.
S. Trifari, G. Sitia, A. Aiuti, S. Scaramuzza, F. Marangoni, L. G. Guidotti, S. Martino, P. Saracco, L. D. Notarangelo, M.-G. Roncarolo, et al. (2006)
J. Immunol. 177, 7451-7461
   Abstract »    Full Text »    PDF »
Regulation of Neuronal Morphology by Toca-1, an F-BAR/EFC Protein That Induces Plasma Membrane Invagination.
T. Kakimoto, H. Katoh, and M. Negishi (2006)
J. Biol. Chem. 281, 29042-29053
   Abstract »    Full Text »    PDF »
The Yeast Actin Cytoskeleton: from Cellular Function to Biochemical Mechanism.
J. B. Moseley and B. L. Goode (2006)
Microbiol. Mol. Biol. Rev. 70, 605-645
   Abstract »    Full Text »    PDF »
Tuba stimulates intracellular N-WASP-dependent actin assembly.
E. M. Kovacs, R. S. Makar, and F. B. Gertler (2006)
J. Cell Sci. 119, 2715-2726
   Abstract »    Full Text »    PDF »
Interaction of N-WASP with hnRNPK and Its Role in Filopodia Formation and Cell Spreading.
Y. Yoo, X. Wu, C. Egile, R. Li, and J.-L. Guan (2006)
J. Biol. Chem. 281, 15352-15360
   Abstract »    Full Text »    PDF »
Characterization of the megakaryocyte demarcation membrane system and its role in thrombopoiesis.
H. Schulze, M. Korpal, J. Hurov, S.-W. Kim, J. Zhang, L. C. Cantley, T. Graf, and R. A. Shivdasani (2006)
Blood 107, 3868-3875
   Abstract »    Full Text »    PDF »
CARMIL Is a Potent Capping Protein Antagonist: IDENTIFICATION OF A CONSERVED CARMIL DOMAIN THAT INHIBITS THE ACTIVITY OF CAPPING PROTEIN AND UNCAPS CAPPED ACTIN FILAMENTS.
T. Uruno, K. Remmert, and J. A. Hammer III (2006)
J. Biol. Chem. 281, 10635-10650
   Abstract »    Full Text »    PDF »
Thin layer chromatography-blotting, a novel method for the detection of phosphoinositides..
M. Furutani, T. Itoh, T. Ijuin, K. Tsujita, and T. Takenawa (2006)
J. Biochem. 139, 663-670
   Abstract »    Full Text »    PDF »
Protein kinase A-dependent increase in WAVE2 expression induced by the focal adhesion protein vinexin.
M. Mitsushima, T. Sezaki, R. Akahane, K. Ueda, S. Suetsugu, T. Takenawa, and N. Kioka (2006)
Genes Cells 11, 281-292
   Abstract »    Full Text »    PDF »
Role of the WASP family proteins for Mycobacterium marinum actin tail formation.
L. M. Stamm, M. A. Pak, J. H. Morisaki, S. B. Snapper, K. Rottner, S. Lommel, and E. J. Brown (2005)
PNAS 102, 14837-14842
   Abstract »    Full Text »    PDF »
A WASp-binding type II phosphatidylinositol 4-kinase required for actin polymerization-driven endosome motility.
F. S. Chang, G.-S. Han, G. M. Carman, and K. J. Blumer (2005)
J. Cell Biol. 171, 133-142
   Abstract »    Full Text »    PDF »
The leading edge is a lipid diffusion barrier.
I. Weisswange, T. Bretschneider, and K. I. Anderson (2005)
J. Cell Sci. 118, 4375-4380
   Abstract »    Full Text »    PDF »
Regulation of Actin Ring Formation by Rho GTPases in Osteoclasts.
M. A. Chellaiah (2005)
J. Biol. Chem. 280, 32930-32943
   Abstract »    Full Text »    PDF »
WAVE2 Signaling Mediates Invasion of Polarized Epithelial Cells by Salmonella typhimurium.
J. Shi, G. Scita, and J. E. Casanova (2005)
J. Biol. Chem. 280, 29849-29855
   Abstract »    Full Text »    PDF »
Ligation of Cell Surface-Associated Glucose-Regulated Protein 78 by Receptor-Recognized Forms of {alpha}2-Macroglobulin: Activation of p21-Activated Protein Kinase-2-Dependent Signaling in Murine Peritoneal Macrophages.
U. K. Misra, T. Sharma, and S. V. Pizzo (2005)
J. Immunol. 175, 2525-2533
   Abstract »    Full Text »    PDF »
Genotype-Proteotype Linkage in the Wiskott-Aldrich Syndrome.
M. I. Lutskiy, F. S. Rosen, and E. Remold-O'Donnell (2005)
J. Immunol. 175, 1329-1336
   Abstract »    Full Text »    PDF »
The Adapter Protein CrkII Regulates Neuronal Wiskott-Aldrich Syndrome Protein, Actin Polymerization, and Tension Development during Contractile Stimulation of Smooth Muscle.
D. D. Tang, W. Zhang, and S. J. Gunst (2005)
J. Biol. Chem. 280, 23380-23389
   Abstract »    Full Text »    PDF »
WAVE3-mediated Cell Migration and Lamellipodia Formation Are Regulated Downstream of Phosphatidylinositol 3-Kinase.
K. Sossey-Alaoui, X. Li, T. A. Ranalli, and J. K. Cowell (2005)
J. Biol. Chem. 280, 21748-21755
   Abstract »    Full Text »    PDF »
Activation of the Arp2/3 complex by N-WASp is required for actin polymerization and contraction in smooth muscle.
W. Zhang, Y. Wu, L. Du, D. D. Tang, and S. J. Gunst (2005)
Am J Physiol Cell Physiol 288, C1145-C1160
   Abstract »    Full Text »    PDF »
A Dictyostelium Homologue of WASP Is Required for Polarized F-Actin Assembly during Chemotaxis.
S. A. Myers, J. W. Han, Y. Lee, R. A. Firtel, and C. Y. Chung (2005)
Mol. Biol. Cell 16, 2191-2206
   Abstract »    Full Text »    PDF »
Two Distinct Head-Tail Interfaces Cooperate to Suppress Activation of Vinculin by Talin.
D. M. Cohen, H. Chen, R. P. Johnson, B. Choudhury, and S. W. Craig (2005)
J. Biol. Chem. 280, 17109-17117
   Abstract »    Full Text »    PDF »
The nucleotide switch in Cdc42 modulates coupling between the GTPase-binding and allosteric equilibria of Wiskott-Aldrich syndrome protein.
D. W. Leung and M. K. Rosen (2005)
PNAS 102, 5685-5690
   Abstract »    Full Text »    PDF »
Phosphatidylinositol-4,5-bisphosphate hydrolysis directs actin remodeling during phagocytosis.
C. C. Scott, W. Dobson, R. J. Botelho, N. Coady-Osberg, P. Chavrier, D. A. Knecht, C. Heath, P. Stahl, and S. Grinstein (2005)
J. Cell Biol. 169, 139-149
   Abstract »    Full Text »    PDF »
The NF2 Tumor Suppressor Merlin and the ERM Proteins Interact with N-WASP and Regulate Its Actin Polymerization Function.
N. Manchanda, A. Lyubimova, H.-Y. H. Ho, M. F. James, J. F. Gusella, N. Ramesh, S. B. Snapper, and V. Ramesh (2005)
J. Biol. Chem. 280, 12517-12522
   Abstract »    Full Text »    PDF »
DISTORTED3/SCAR2 Is a Putative Arabidopsis WAVE Complex Subunit That Activates the Arp2/3 Complex and Is Required for Epidermal Morphogenesis.
D. Basu, J. Le, S. E.-D. El-Essal, S. Huang, C. Zhang, E. L. Mallery, G. Koliantz, C. J. Staiger, and D. B. Szymanski (2005)
PLANT CELL 17, 502-524
   Abstract »    Full Text »    PDF »
The role of the Rho GTPases in neuronal development.
E.-E. Govek, S. E. Newey, and L. Van Aelst (2005)
Genes & Dev. 19, 1-49
   Abstract »    Full Text »    PDF »
Nak1 interacts with Hob1 and Wsp1 to regulate cell growth and polarity in Schizosaccharomyces pombe.
T. Y. Huang, M. Renaud-Young, and D. Young (2005)
J. Cell Sci. 118, 199-210
   Abstract »    Full Text »    PDF »
Cortactin regulates cell migration through activation of N-WASP.
J. R. Kowalski, C. Egile, S. Gil, S. B. Snapper, R. Li, and S. M. Thomas (2005)
J. Cell Sci. 118, 79-87
   Abstract »    Full Text »    PDF »
The Small GTPase Cdc42 Regulates Actin Polymerization and Tension Development during Contractile Stimulation of Smooth Muscle.
D. D. Tang and S. J. Gunst (2004)
J. Biol. Chem. 279, 51722-51728
   Abstract »    Full Text »    PDF »
Mechanisms of WASp-mediated hematologic and immunologic disease.
S. Burns, G. O. Cory, W. Vainchenker, and A. J. Thrasher (2004)
Blood 104, 3454-3462
   Abstract »    Full Text »    PDF »
Early signaling events involved in the entry of Rickettsia conorii into mammalian cells.
J. J. Martinez and P. Cossart (2004)
J. Cell Sci. 117, 5097-5106
   Abstract »    Full Text »    PDF »
Interchangeable functions of Arabidopsis PIROGI and the human WAVE complex subunit SRA1 during leaf epidermal development.
D. Basu, S. E.-D. El-Assal, J. Le, E. L. Mallery, and D. B. Szymanski (2004)
Development 131, 4345-4355
   Abstract »    Full Text »    PDF »
The Activity of the Plexin-A1 Receptor Is Regulated by Rac.
L. J. Turner, S. Nicholls, and A. Hall (2004)
J. Biol. Chem. 279, 33199-33205
   Abstract »    Full Text »    PDF »
Cdc42, Rac1, and Rac2 Display Distinct Patterns of Activation during Phagocytosis.
A. D. Hoppe and J. A. Swanson (2004)
Mol. Biol. Cell 15, 3509-3519
   Abstract »    Full Text »    PDF »
Caenorhabditis elegans WASP and Ena/VASP Proteins Play Compensatory Roles in Morphogenesis and Neuronal Cell Migration.
J. Withee, B. Galligan, N. Hawkins, and G. Garriga (2004)
Genetics 167, 1165-1176
   Abstract »    Full Text »    PDF »
Erk/Src Phosphorylation of Cortactin Acts as a Switch On-Switch Off Mechanism That Controls Its Ability To Activate N-WASP.
N. Martinez-Quiles, H.-Y. H. Ho, M. W. Kirschner, N. Ramesh, and R. S. Geha (2004)
Mol. Cell. Biol. 24, 5269-5280
   Abstract »    Full Text »    PDF »
Regulated Membrane Trafficking of the Insulin-Responsive Glucose Transporter 4 in Adipocytes.
R. T. Watson, M. Kanzaki, and J. E. Pessin (2004)
Endocr. Rev. 25, 177-204
   Abstract »    Full Text »    PDF »
EHD2 and the Novel EH Domain Binding Protein EHBP1 Couple Endocytosis to the Actin Cytoskeleton.
A. Guilherme, N. A. Soriano, S. Bose, J. Holik, A. Bose, D. P. Pomerleau, P. Furcinitti, J. Leszyk, S. Corvera, and M. P. Czech (2004)
J. Biol. Chem. 279, 10593-10605
   Abstract »    Full Text »    PDF »
Cascade pathway of filopodia formation downstream of SCAR.
A. Biyasheva, T. Svitkina, P. Kunda, B. Baum, and G. Borisy (2004)
J. Cell Sci. 117, 837-848
   Abstract »    Full Text »    PDF »
Regulated Exocytosis in Neuroendocrine Cells: A Role for Subplasmalemmal Cdc42/N-WASP-induced Actin Filaments.
S. Gasman, S. Chasserot-Golaz, M. Malacombe, M. Way, and M.-F. Bader (2004)
Mol. Biol. Cell 15, 520-531
   Abstract »    Full Text »    PDF »
From the Cover: Visualization of spatially and temporally regulated N-WASP activity during cytoskeletal reorganization in living cells.
M. E. Ward, J. Y. Wu, and Y. Rao (2004)
PNAS 101, 970-974
   Abstract »    Full Text »    PDF »
Tuba, a Novel Protein Containing Bin/Amphiphysin/Rvs and Dbl Homology Domains, Links Dynamin to Regulation of the Actin Cytoskeleton.
M. A. Salazar, A. V. Kwiatkowski, L. Pellegrini, G. Cestra, M. H. Butler, K. L. Rossman, D. M. Serna, J. Sondek, F. B. Gertler, and P. De Camilli (2003)
J. Biol. Chem. 278, 49031-49043
   Abstract »    Full Text »    PDF »
Translocation of N-WASP by Nuclear Localization and Export Signals into the Nucleus Modulates Expression of HSP90.
S. Suetsugu and T. Takenawa (2003)
J. Biol. Chem. 278, 42515-42523
   Abstract »    Full Text »    PDF »
Reprogramming Control of an Allosteric Signaling Switch Through Modular Recombination.
J. E. Dueber, B. J. Yeh, K. Chak, and W. A. Lim (2003)
Science 301, 1904-1908
   Abstract »    Full Text »    PDF »
Regulation of Actin Dynamics by WASP Family Proteins.
H. Miki and T. Takenawa (2003)
J. Biochem. 134, 309-313
   Abstract »    Full Text »    PDF »
Arf6 and Phosphoinositol-4-Phosphate-5-Kinase Activities Permit Bypass of the Rac1 Requirement for {beta}1 Integrin-mediated Bacterial Uptake.
K.-W. Wong and R. R. Isberg (2003)
J. Exp. Med. 198, 603-614
   Abstract »    Full Text »    PDF »
WIP participates in actin reorganization and ruffle formation induced by PDGF.
I. M. Anton, S. P. Saville, M. J. Byrne, C. Curcio, N. Ramesh, J. H. Hartwig, and R. S. Geha (2003)
J. Cell Sci. 116, 2443-2451
   Abstract »    Full Text »    PDF »
Membrane Ruffling Requires Coordination between Type I{alpha} Phosphatidylinositol Phosphate Kinase and Rac Signaling.
R. L. Doughman, A. J. Firestone, M. L. Wojtasiak, M. W. Bunce, and R. A. Anderson (2003)
J. Biol. Chem. 278, 23036-23045
   Abstract »    Full Text »    PDF »
Gene therapy for Wiskott-Aldrich syndrome: rescue of T-cell signaling and amelioration of colitis upon transplantation of retrovirally transduced hematopoietic stem cells in mice.
C. Klein, D. Nguyen, C.-H. Liu, A. Mizoguchi, A. K. Bhan, H. Miki, T. Takenawa, F. S. Rosen, F. W. Alt, R. C. Mulligan, et al. (2003)
Blood 101, 2159-2166
   Abstract »    Full Text »    PDF »
Effects of Ectopically Expressed Neuronal Wiskott-Aldrich Syndrome Protein Domains on Rickettsia rickettsii Actin-Based Motility.
R. S. Harlander, M. Way, Q. Ren, D. Howe, S. S. Grieshaber, and R. A. Heinzen (2003)
Infect. Immun. 71, 1551-1556
   Abstract »    Full Text »    PDF »
Neural Wiskott-Aldrich Syndrome Protein Is Recruited to Rafts and Associates with Endophilin A in Response to Epidermal Growth Factor.
M. Otsuki, T. Itoh, and T. Takenawa (2003)
J. Biol. Chem. 278, 6461-6469
   Abstract »    Full Text »    PDF »
EPLIN regulates actin dynamics by cross-linking and stabilizing filaments.
R. S. Maul, Y. Song, K. J. Amann, S. C. Gerbin, T. D. Pollard, and D. D. Chang (2003)
J. Cell Biol. 160, 399-407
   Abstract »    Full Text »    PDF »
Mechanism of filopodia initiation by reorganization of a dendritic network.
T. M. Svitkina, E. A. Bulanova, O. Y. Chaga, D. M. Vignjevic, S.-i. Kojima, J. M. Vasiliev, and G. G. Borisy (2003)
J. Cell Biol. 160, 409-421
   Abstract »    Full Text »    PDF »
A Role for the Juxtamembrane Domain of beta -Dystroglycan in Agrin-Induced Acetylcholine Receptor Clustering.
J. Kahl and J. T. Campanelli (2003)
J. Neurosci. 23, 392-402
   Abstract »    Full Text »    PDF »
Actin cytoskeletal dynamics in T lymphocyte activation and migration.
Y. Samstag, S. M. Eibert, M. Klemke, and G. H. Wabnitz (2003)
J. Leukoc. Biol. 73, 30-48
   Abstract »    Full Text »    PDF »
Recruitment of the Arp2/3 complex to vinculin: coupling membrane protrusion to matrix adhesion.
K. A. DeMali, C. A. Barlow, and K. Burridge (2002)
J. Cell Biol. 159, 881-891
   Abstract »    Full Text »    PDF »
Signal transduction during Fc receptor-mediated phagocytosis.
E. Garcia-Garcia and C. Rosales (2002)
J. Leukoc. Biol. 72, 1092-1108
   Abstract »    Full Text »    PDF »
Nogo-A and Myelin-Associated Glycoprotein Mediate Neurite Growth Inhibition by Antagonistic Regulation of RhoA and Rac1.
B. Niederost, T. Oertle, J. Fritsche, R. A. McKinney, and C. E. Bandtlow (2002)
J. Neurosci. 22, 10368-10376
   Abstract »    Full Text »    PDF »
Phosphorylation of Tyrosine 291 Enhances the Ability of WASp to Stimulate Actin Polymerization and Filopodium Formation.
G. O. C. Cory, R. Garg, R. Cramer, and A. J. Ridley (2002)
J. Biol. Chem. 277, 45115-45121
   Abstract »    Full Text »    PDF »
Novel membrane traffic steps regulate the exocytosis of the Menkes disease ATPase.
C. Cobbold, S. Ponnambalam, M. J. Francis, and A. P. Monaco (2002)
Hum. Mol. Genet. 11, 2855-2866
   Abstract »    Full Text »    PDF »
Motility Determinants in WASP Family Proteins.
D. Yarar, J. A. D'Alessio, R. L. Jeng, and M. D. Welch (2002)
Mol. Biol. Cell 13, 4045-4059
   Abstract »    Full Text »    PDF »
Pairs of dipeptides synergistically activate the binding of substrate by ubiquitin ligase through dissociation of its autoinhibitory domain.
F. Du, F. Navarro-Garcia, Z. Xia, T. Tasaki, and A. Varshavsky (2002)
PNAS 99, 14110-14115
   Abstract »    Full Text »    PDF »
Phosphatidylinositol 4,5-Biphosphate (PIP2)-induced Vesicle Movement Depends on N-WASP and Involves Nck, WIP, and Grb2.
S. Benesch, S. Lommel, A. Steffen, T. E. B. Stradal, N. Scaplehorn, M. Way, J. Wehland, and K. Rottner (2002)
J. Biol. Chem. 277, 37771-37776
   Abstract »    Full Text »    PDF »
Lateral Sequestration of Phosphatidylinositol 4,5-Bisphosphate by the Basic Effector Domain of Myristoylated Alanine-rich C Kinase Substrate Is Due to Nonspecific Electrostatic Interactions.
J. Wang, A. Gambhir, G. Hangyas-Mihalyne, D. Murray, U. Golebiewska, and S. McLaughlin (2002)
J. Biol. Chem. 277, 34401-34412
   Abstract »    Full Text »    PDF »
Localization of Receptor-Mediated Signal Transduction Pathways: The Inside Story.
J. M. Haugh (2002)
Mol. Interv. 2, 292-307
   Abstract »    Full Text »    PDF »
Normal Arp2/3 complex activation in platelets lacking WASp.
H. Falet, K. M. Hoffmeister, R. Neujahr, and J. H. Hartwig (2002)
Blood 100, 2113-2122
   Abstract »    Full Text »    PDF »
Neural Wiskott-Aldrich Syndrome Protein Is Involved in Hepatocyte Growth Factor-induced Migration, Invasion, and Tubulogenesis of Epithelial Cells.
H. Yamaguchi, H. Miki, and T. Takenawa (2002)
Cancer Res. 62, 2503-2509
   Abstract »    Full Text »    PDF »
Nir2, a Novel Regulator of Cell Morphogenesis.
D. Tian, V. Litvak, M. Toledo-Rodriguez, S. Carmon, and S. Lev (2002)
Mol. Cell. Biol. 22, 2650-2662
   Abstract »    Full Text »    PDF »
Enhancement of branching efficiency by the actin filament-binding activity of N-WASP/WAVE2.
S. Suetsugu, H. Miki, H. Yamaguchi, T. Obinata, and T. Takenawa (2002)
J. Cell Sci. 114, 4533-4542
   Abstract »    Full Text »    PDF »